Denisa Chládková (born February 8, 1979 in Prague, Czech Republic) is a professional tennis player from the Czech Republic.

She is yet to win any titles, but she is probably best remembered for reaching the Wimbledon quarterfinals in 1997, playing in only her third Grand Slam main draw. In the second round, she stunned Lindsay Davenport for the first top ten win of her career, but eventually lost to the champion Martina Hingis.

Despite not winning any titles, she has reached WTA Tour singles finals. The biggest of these was at the Tier II event held in Hannover, Germany, where she lost to Serena Williams. She also finished runner-up at Knokke-Heist, losing to Maria Sanchez Lorenzo and Helsinki, losing to Svetlana Kuznetsova.

On June 16, 2003, she ascended to her career high ranking of No. 31 in the world. That same year she advanced to the fourth round of the Australian Open, her best Grand Slam result since her memorable Wimbledon quarterfinal run six years previously.

During her career, she has earned US$1,343,285 and has wins over Lindsay Davenport, Barbara Schett, Anke Huber, Chanda Rubin, Silvia Farina Elia, Tamarine Tanasugarn and Magdalena Maleeva.